Isolation, Characterization and Sub-acute Toxicity Studies of a New Compound PITC-2 Isolated from Tissue-Cultured Medicinal Plant, Pluchea indica (L.) Less
نویسندگان
چکیده
The present study focused on the chemical characterization of PITC-2 (2-(prop-1-ynyl)-5(5,6-dihydroxyhexa-1, 3-diynyl)-thiophene, a thiophen derivative) isolated from the root extract of tissue-cultured medicinal plant Pluchea indica (L.) Less. Evaluation of sub-acute toxicity study of the new compound was also done in vivo. Methanolic root extract of tissue-cultured P. indica was separated by solventsolvent partition into three fractions (water, n-butanol, and ethyl-acetate). The ethyl-acetate soluble fraction was subjected to column chromatography using silica gel and PITC-2 was isolated and characterized by NMR IR, and MS. Sub-acute toxicity study of PITC-2 was studied on Swiss Wistar rats. The studies included the gross observation such as changes in body weight, hematological profiles [total count of red blood cell (RBC), white blood cell (WBC) and differential count of white blood cell and haemoglobin (Hb) percentage], biochemical parameters of serum [total protein, serum glutamate oxaloacetate transminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phosphatase (SALP), serum bilirubin, creatinine, urea, triglyceride, cholesterol, glucose], liver enzymes level [thiobarbituric acid reactive substance (TBARS), glutathione (GSH), super oxide dismutage (SOD)] and histopathology of the liver, kidney, heart, spleen and lung of both the control and experimental groups of rats. The changes of body weight, hematological profiles, biochemical parameter, and liver enzymes were not significant compared to control groups of animals. However, a change of liver weight but not of hepatic enzymes was observed. _____________________________________________________________________________________________________________
منابع مشابه
In Vitro Antimicrobial Activity of Thiophene Derivative Pitc-2 of Pluchea Indica and Its Mechanism of Action
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